abstract：:The cloning of fga1, the gene encoding a G protein alpha subunit, was performed by standard PCR techniques and by screening a Fusarium oxysporum genomic library, using the PCR product as a probe. The full-length open reading frame spanned 1,059 nucleotides and the deduced primary structure of the protein (353 amino ac...

journal_title：Current genetics

authors： Jain S,Akiyama K,Mae K,Ohguchi T,Takata R

更新日期：2002-09-01 00:00:00

abstract：:Schizosaccharomyces pombe Cdc23 is an essential DNA replication protein, conserved in eukaryotes and functionally homologous with Saccharomyces cerevisiae Dna43 (Mcm10). We sought evidence for interactions between Cdc23 and the MCM2-7 complex, a component of both the pre-replicative complex and the replication fork. C...

journal_title：Current genetics

authors： Hart EA,Bryant JA,Moore K,Aves SJ

更新日期：2002-08-01 00:00:00

abstract：:cAMP-dependent protein kinases (PKA) are ubiquitous signalling molecules that mediate many extracellular signals in eukaryotes from yeast to men. Directing PKA to its substrates is an important level of control to ensure specificity of cAMP-mediated signal transduction. Unlike in yeast and fungi, in mammalian cells a ...

journal_title：Current genetics

authors： Griffioen G,Thevelein JM

更新日期：2002-07-01 00:00:00

abstract：:The potato (Solanum tuberosum L.) mitochondrial cox3/ sdh4 gene cluster was previously identified by heterologous hybridization using a Marchantia polymorpha sdh4 probe. In this work, this potato gene cluster was cloned, sequenced and its expression was evaluated. The gene sequence and gene locus organization were fou...

journal_title：Current genetics

authors： Siqueira SF,Dias SM,Hardouin P,Pereira FR,Lejeune B,de Souza AP

更新日期：2002-07-01 00:00:00

abstract：:Cellulases belong to the large family of glycosyl hydrolases (GHs) and are produced by a variety of bacteria and fungi. These extracellular enzymes act as endoglucanases (EGs), cellobiohydrolases or beta-glucosidases. In this paper, we describe molecular screening for EGs from the GH family 12. Using three homologous ...

journal_title：Current genetics

authors： Goedegebuur F,Fowler T,Phillips J,van der Kley P,van Solingen P,Dankmeyer L,Power SD

更新日期：2002-05-01 00:00:00

abstract：:Primary transcripts in higher plant cell organelles undergo a series of essential RNA processing steps, including 5'- and 3'-terminal maturation, intron splicing and RNA editing. Most of the protein factors involved in these RNA processing mechanisms are still unknown. Also, little is known about the environmental and...

journal_title：Current genetics

authors： Karcher D,Bock R

更新日期：2002-04-01 00:00:00

abstract：:A gene encoding mitochondrial S13 is generally present in the mitochondrial genome of higher plants, but is lacking from the Arabidopsis thaliana mitochondrial genome. Previous research has failed to identify a nuclear gene capable of encoding a mitochondrial S13 protein or the protein itself. Doubts have even been ra...

journal_title：Current genetics

authors： Mollier P,Hoffmann B,Debast C,Small I

更新日期：2002-03-01 00:00:00

abstract：:The microtubule-organizing center (MTOC) is the organelle that manages the distribution of microtubule filaments in the cell. It includes such centers of microtubule organization as basal bodies, nucleus-associated bodies, spindle pole bodies and centrosomes. The centrosome is the MTOC in most animal cells. Despite th...

journal_title：Current genetics

authors： Helfant AH

更新日期：2002-02-01 00:00:00

abstract：:The mammalian HBP2 gene has been isolated by functional complementation of cells unable to undergo DNA replication in fission yeast. HBP2 is a protein with a high mobility group (HMG) domain that belongs to the Sox (Sry-related HMG box) family of transcription factors. As in other members of the family, the HMG box in...

journal_title：Current genetics

authors： Sánchez-Díaz A,Blanco MA,Jones N,Moreno S

更新日期：2001-09-01 00:00:00

abstract：:How group I introns originate in nuclear ribosomal (r)RNA genes is an important question in evolutionary biology. Central to this issue is the multitude of group I introns present in evolutionarily distantly related plant, fungal, and protist lineages, together with an understanding of their origin and lateral transfe...

journal_title：Current genetics

authors： Bhattacarya D,Cannone JJ,Gutell RR

更新日期：2001-08-01 00:00:00

abstract：:Tuplp is a general corepressor in Saccharomyces cerevisiae. We performed a split-ubiquitin screen with Tup1p as bait, in order to understand how Tuplp mediates repression. Cdc73p was the only component of a holoenzyme of transcription isolated as a Tuplp-interacting protein in this screen. Expression analysis of a str...

journal_title：Current genetics

authors： Kerkmann K,Lehming N

更新日期：2001-07-01 00:00:00

abstract：:The circular mitochondrial genome of Dictyostelium discoideum has a size of 55,564 base pairs. We present here a complete and detailed transcription map of the mitochondrial DNA. Eight major, polycistronic transcripts encoding polypeptides, ribosomal RNAs and interspersed transfer RNAs were identified in Northern hybr...

journal_title：Current genetics

authors： Barth C,Greferath U,Kotsifas M,Tanaka Y,Alexander S,Alexander H,Fisher PR

更新日期：2001-07-01 00:00:00

abstract：:The RNA polymerase II (Pol II) of Schizosaccharomyces pombe is composed of 12 subunits. Subunit Rpb3 has sequence homology with the N-terminal domain of the prokaryotic alpha subunit, which plays a key role in RNA polymerase assembly. Together with the Rpb2 (the beta homologue) and Rpb11 (the second alpha homologue) s...

journal_title：Current genetics

authors： Mitobe J,Mitsuzawa H,Ishihama A

更新日期：2001-06-01 00:00:00

abstract：:We report the isolation and expression profiles of a single-copy gene from the mycoparasitic fungus Trichoderma hamatum encoding a 60 S cytoplasmic ribosomal protein. The gene, named RPL36, was cloned through its nutrient-mediated expression, using mRNA differential screening. Its predicted ORF, interrupted by two int...

journal_title：Current genetics

authors： Fekete C,Posta K,Hornok L

更新日期：2001-05-01 00:00:00

abstract：:Non-LTR retrotransposons (also known as LINEs) have had a major influence on the structure of many eukaryote genomes. They are abundant in many multicellular eukaryotes, including mammals, but appear to be absent from the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. This absence has, to date, preclud...

journal_title：Current genetics

authors： Goodwin TJ,Ormandy JE,Poulter RT

更新日期：2001-04-01 00:00:00

abstract：:The gene encoding endopolygalacturonase (EC 3.2.1.15) has been cloned, sequenced and expressed from three strains of Saccharomyces cerevisiae (including nonsecretors) and three strains of Kluyveromyces marxianus. Both control and coding regions showed small differences within each species, one including loss of a pote...

journal_title：Current genetics

authors： Jia J,Wheals A

更新日期：2000-12-01 00:00:00

abstract：:Mitochondrial (mt) DNA structures were studied in 12 nucleus-cytoplasm (NC) hybrids of tetraploid and hexaploid wheat in which nuclear genomes of the recurrent paternal wheat parents were combined with D and D2 plasmons from Aegilops species. RFLP analysis of 15 mtDNA regions indicated the presence of the paternal seq...

journal_title：Current genetics

authors： Tsukamoto N,Asakura N,Hattori N,Takumi S,Mori N,Nakamura C

更新日期：2000-11-01 00:00:00

abstract：:The het-c locus controls heterokaryon formation in Neurospora crassa. It is subject to balancing selection operating to maintain polymorphism at that locus in natural populations. We have isolated hch, the het-c homolog from the related species Podospora anserina (hch for het-c homolog), in order to determine if this ...

journal_title：Current genetics

authors： Saupe SJ,Clavé C,Sabourin M,Bégueret J

更新日期：2000-07-01 00:00:00

abstract：:The regulation of dextranase (dexA) gene expression in the filamentous fungus Penicillium minioluteum grown on different carbon sources was studied. Growth in the presence of dextran leads to high expression of the dextranase enzyme, but growth in starch, glucose, glycerol, lactose and sorbitol did not. Dextran induce...

journal_title：Current genetics

authors： García B,Rodríguez E

更新日期：2000-06-01 00:00:00

abstract：:In filamentous ascomycetes, glucose repression is mediated by CRE1, a zinc-finger protein related to Miglp from yeast. Five putative AMPK phosphorylation motifs identified in the glucose repressor from the phytopathogenic fungus Sclerotinia sclerotiorum were mutated in a GFP::CRE1 translational fusion. Complementation...

journal_title：Current genetics

authors： Vautard-Mey G,Fèvre M

更新日期：2000-05-01 00:00:00

abstract：:In the photosynthetic chloroplast mutant PRB2A of Chlamydomonas reinhardtii the psbD mRNA is unstable. Three strains were isolated, in which the underlying site-directed mutation within the psbD 5' UTR (untranslated region) is suppressed. In all three suppressors, psbD RNA levels and RNA 5' maturation are restored to ...

journal_title：Current genetics

authors： Nickelsen J

更新日期：2000-02-01 00:00:00

abstract：:Degenerative processes in the filamentous fungus Podospora anserina are strongly correlated with the instability of the mitochondrial genome. Among the sources of instability is the mobile group-II intron COX1-i1, also called intron alpha, which encodes a protein with a reverse transcriptase activity. In this paper we...

journal_title：Current genetics

authors： Sellem CH,Begel O,Sainsard-Chanet A

更新日期：2000-01-01 00:00:00

abstract：:In Volvox carteri, regA acts as a master gene to suppress all germ cell functions in somatic cells. Its product, RegA, has features of a transcriptional repressor. Here we report cDNA sequences representing 15 nuclear genes with properties expected of RegA targets: they are expressed strongly in germ cells and in regA...

journal_title：Current genetics

authors： Meissner M,Stark K,Cresnar B,Kirk DL,Schmitt R

更新日期：1999-12-01 00:00:00

abstract：:In previous work, four genes involved in mating-type determination were cloned from reference strains of Pyrenopeziza brassicae; three genes, PAD1, PMT1, and PHB1 (re-named henceforth as MAT-1-1, MAT-1-4, and MAT1-3, respectively), are encoded by the MAT-1 idiomorph, and one gene, PHB2 (re-named MAT-2), by the corresp...

journal_title：Current genetics

authors： Singh G,Dyer PS,Ashby AM

更新日期：1999-11-01 00:00:00

abstract：:To gain a deeper insight into the evolution of mating-type genes from filamentous ascomycetes, a comprehensive sequence analysis of PCR-amplified sequences corresponding to A- and a-specific mating-type sequences was undertaken. The study included nine homothallic (compatible) and eight heterothallic (incompatible) me...

journal_title：Current genetics

authors： Pöggeler S

更新日期：1999-10-01 00:00:00

abstract：:Chloroplast genes are typically organized into polycistronic transcription units that give rise to complex sets of mono- and oligo-cistronic overlapping RNAs through a series of processing steps. The psbB operon contains genes for the PSII (psbB, psbT, psbH) and cytochrome b(6)f (petB and petD) complexes which are nee...

journal_title：Current genetics

authors： Dixit R,Trivedi PK,Nath P,Sane PV

更新日期：1999-09-01 00:00:00

abstract：:The RAD52 group of genes in the yeast Saccharomyces cerevisiae controls the repair of DNA damage by a recombinational mechanism. Despite the growing evidence for physical and biochemical interactions between the proteins of this repair group, mutations in individual genes show very different effects on various types o...

journal_title：Current genetics

authors： Jablonovich Z,Liefshitz B,Steinlauf R,Kupiec M

更新日期：1999-08-01 00:00:00

abstract：:Bands of dsRNA were detected in five out of seven isolates of the entomopathogenic fungus Metarhizium flavoviride. The identity of these bands was proven by RNase and S1-nuclease treatments. The transference of dsRNA between isolates (from CG291 to CG442) was successfully carried out through forced heterokaryons. Isog...

journal_title：Current genetics

authors： Martins MK,Furlaneto MC,Sosa-Gomez DR,Faria MR,Fungaro MH

更新日期：1999-08-01 00:00:00

abstract：:There are at least ten plant diseases caused by Alternaria species in which host-specific toxins (HSTs) are responsible for fungal pathogenicity. Of these HST-producers, seven are considered distinct pathotypes of the species Alternaria alternata, and the remaining three are among other species of pathogenic Alternari...

journal_title：Current genetics

authors： Akamatsu H,Taga M,Kodama M,Johnson R,Otani H,Kohmoto K

更新日期：1999-07-01 00:00:00

abstract：:Cenococcum geophilum is an ecologically important mycorrhizal fungus with a global distribution and a wide host range. It has been difficult to study since it forms only sterile mycelia and, occasionally, sclerotial bodies. Because of its lack of morphological variability, its taxonomy and phylogenetic origins have un...

journal_title：Current genetics

authors： Shinohara ML,LoBuglio KF,Rogers SO

更新日期：1999-06-01 00:00:00

abstract：:The ornithine transaminase (otaA) gene of Aspergillus nidulans has been cloned by transformation of the A. nidulans pro-ota- mutant strain with a cosmid gene library. The otaA gene contains two introns and potentially codes for a 453-aa-long protein. The deduced amino-acid sequence is homologous to known ornithine tra...

journal_title：Current genetics

authors： Dzikowska A,Swianiewicz M,Talarczyk A,Wisniewska M,Goras M,Scazzocchio C,Weglenski P

更新日期：1999-03-01 00:00:00

abstract：:The Cercospora nicotianae SOR1 gene is required for resistance to singlet oxygen-generating photosensitizers. SOR1 was characterized in the wild-type and in five photosensitizer-sensitive mutant strains which are complemented to photosensitizer resistance by transformation with SOR1. Sequence analysis determined that ...

journal_title：Current genetics

authors： Ehrenshaft M,Chung KR,Jenns AE,Daub ME

更新日期：1999-01-01 00:00:00

abstract：:The putative gene encoding acetyl-CoA carboxylase, accA, has been isolated from Aspergillus nidulans. This single-copy gene has an open reading frame (ORF) of 6864 bp and contains two small introns near the 5'-end. A short ORF upstream of the ATG start codon has been identified in this gene by RT-PCR. Based on sequenc...

journal_title：Current genetics

authors： Morrice J,MacKenzie DA,Parr AJ,Archer DB

更新日期：1998-12-01 00:00:00

abstract：:Spontaneous and double-strand break (DSB)-induced gene conversion in Saccharomyces cerevisiae was assayed using non-tandem chromosomal direct repeat crosses and plasmid x chromosome crosses. Each cross involved identical ura3 alleles marked with phenotypically silent restriction fragment length polymorphic (RFLP) muta...

journal_title：Current genetics

authors： Cho JW,Khalsa GJ,Nickoloff JA

更新日期：1998-10-01 00:00:00

abstract：:Some diploid strains of Saccharomyces cerevisiae can grow both as a spherical yeast form and as a filamentous pseudohyphal form. Most yeasts capable of forming pseudohyphae possess a functional FLO8 gene. We show that disrupting the ACE2 transcription factor results in the production of pseudohyphae in a flo8-1 backgr...

journal_title：Current genetics

authors： King L,Butler G

更新日期：1998-09-01 00:00:00

abstract：:ERG24 is the structural gene for the C14-sterol reductase in yeast. A lack of activity in that enzyme, mediated either by the morpholine fungicides or the insertional inactivation of ERG24, causes the accumulation of the aberrant sterol ignosterol. Cells producing this sterol are unable to grow aerobically in the rout...

journal_title：Current genetics

authors： Crowley JH,Tove S,Parks LW

更新日期：1998-08-01 00:00:00

abstract：:The Hansenula polymorpha per6-210 mutant is impaired in respect of growth on methanol (Mut-) and is characterized by aberrant peroxisome formation. The functionally complementing DNA fragment contains two open reading frames. The first encodes dihydroxyacetone kinase (DAK), a cytosolic enzyme essential for formaldehyd...

journal_title：Current genetics

authors： van der Klei IJ,van der Heide M,Baerends RJ,Rechinger KB,Nicolay K,Kiel JA,Veenhuis M

更新日期：1998-07-01 00:00:00

abstract：:Ime1 plays a pivotal role in the initiation of meiosis in a/alpha diploid cells of Saccharomyces cerevisiae. In the absence of glucose and nitrogen, IME1 expression is greater in a/alpha cells than in either a or alpha cells and therefore only a/alpha, but not a/a or alpha/alpha, cells are committed to sporulation. It...

journal_title：Current genetics

authors： Mizuno T,Nakazawa N,Remgsamrarn P,Kunoh T,Oshima Y,Harashima S

更新日期：1998-04-01 00:00:00

abstract：:A delta-9 fatty acid desaturase gene, homologous to animal and fungal acyl-coenzyme A (CoA) desaturases, was isolated from the red alga Cyanidioschyzon merolae using a degenerate PCR strategy. This gene, designated as CmFAD9, has no intron. The encoded delta-9 fatty acid desaturase (CmFad9p) consists of 476 amino acid...

journal_title：Current genetics

authors： Itoh R,Toda K,Takahashi H,Takano H,Kuroiwa T

更新日期：1998-03-01 00:00:00

abstract：:Small insertions are reported from the SSU rDNA of the genera Cladonia, Cladina, Stereocaulon, Pertusaria and Physcia (lichen-forming Lecanorales, Ascomycetes). The insertions range in length from 56 to 81 nucleotides, and occur at a shared position, 330 (relative to Escherichia coli), in a semi-conserved region of th...

journal_title：Current genetics

authors： Stenroos S,DePriest PT

更新日期：1998-02-01 00:00:00